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How Third-Party COAs Are Generated: Interpreting Peptide Test Results

A practical, research-focused guide to reading Certificates of Analysis (COAs) with a real Vanguard Laboratory example. For laboratory research use only.

Research Use Only: This article is educational and summarizes analytical testing concepts. It is not medical or clinical guidance and is not intended for human or veterinary use.

A COA (Certificate of Analysis) is a third-party analytical report that documents what was tested, how it was tested, and what the laboratory measured.
At PeptiSearch, we publish COAs so researchers can review identity- and purity-related data directly—without relying on marketing claims.

What a COA Does (and Doesn’t) Tell You

What it documents
Analytical results for the specific submitted sample, using defined lab methods (e.g., HPLC, MS, UV/VIS).
What it does NOT claim
A COA does not establish medical safety, efficacy, dosing, or suitability for any clinical use.
Why it matters
It helps researchers verify identity/purity-related signals and compare batches or vendors using consistent documentation.

Our Independent Lab Partner: Vanguard Laboratory

PeptiSearch uses Vanguard Laboratory for third-party analytical testing. Vanguard is an independent laboratory that provides
method-based reporting (such as chromatographic analysis). Independence matters: it reduces bias and ensures results come from an external source,
not an in-house report.

A Real Example: MOTS-c COA (Lot MOTSC-1125)

Below is an example of what researchers typically review on a COA: product identification details, the analytical method used, and measured results.
This example corresponds to a specific batch (“lot”) and is published so researchers can evaluate the underlying documentation.

COA Field What it means Why it matters
Lot / Batch ID Unique identifier for the tested material Ensures your COA matches the specific product batch being referenced
Method (e.g., HPLC-UV/VIS) Instrument and detection method used to analyze the sample Methods determine what can be detected and how results are calculated
Purity (Chromatographic) Percent of signal attributed to the main analyte under the method A high main peak suggests fewer detectable impurities under that method’s conditions
Quantity / Content Measured amount reported by the lab (method-dependent) Helps researchers compare “label” vs measured content across lots
Uncertainty / Tolerance Reported uncertainty range (when provided) Indicates measurement variability within method and lab conditions

Common COA Misconceptions

Purity ≠ Quantity
A purity percentage describes chromatographic composition under a method. Quantity refers to measured content.
These are different measurements and should be interpreted separately.
COAs are sample-specific
A COA applies to the tested sample and the reported lot. It is not a universal certificate for every batch of a product name.
Method matters
Different methods detect different things. A clean chromatogram under one method does not guarantee absence of all possible impurities.

Why PeptiSearch Publishes Full COAs

  • Verification: Researchers can validate lot-specific documentation directly.
  • Consistency: COAs provide a repeatable reference point across orders and batches.
  • Transparency: We publish the lab’s results as received, without “marketing edits.”
  • Long-term: This COA library grows over time as additional lots are tested.


View COAs & Testing Page

Disclaimer: Content is for laboratory research and educational purposes only. Products are not intended for human or veterinary use, diagnosis, or treatment.